Fig. 3. Adipogenic response in cells treated with different levels of glutamine for 7 days. (A) Representative images of ORO stained cells after 7 days of differentiation treatment with MSO or supplementation with 5, 10, 20 mM glutamine. (B) ORO staining quantitation (arbitrary units). Data analysed using one-way ANOVA, followed by Tukey's post-hoc test and shown as mean ± SEM. Although statistically significant, the difference between control (Ctrl) and glutamine-supplemented AD groups is not presented in graph. Statistical significance was set at p < 0.05, n = 3. Gln: glutamine.